Ribonuclease Inhibitor, RNase inhibitor 40U ul, RI039

Product Description

Source
E. coli strain expressing a recombinant clone that carries Ribonuclease Inhibitor gene from human placenta.
Concentration
40 U/μl.
Unit Definition
One unit is defined as the amount of Recombinant Ribonuclease Inhibitor required inhibit the activity of 5ng of ribonuclease A by 50%.
Activity is measured by the inhibition of hydrolysis of cytidine 2’3’-cyclic monophosphate by ribonuclease A．
Storage Buffer
20mM HEPES-KOH(PH7.6), 50mM KCl, 8mM DTT, 50%(v/v) glycerol
Storage
Store at -20°C
Physical Purity
The purity is ≥95% as judged by SDS-polyacrylamide gel with Coomassie blue staining.
Usage Note
Ribonuclease Inhibitor is active over a broad pH range (pH5.5-9). The standard RT and in vitro transcription uses Ribonuclease Inhibitor at a final concentration of 1u/ul.

Quality Control

Absence of Endonuclease

1μg of Lambda DNA is incubated with 200 units of Ribonuclease Inhibitor for 16 hours at 37°C.
Following incubation, Lambda DNA is visualized as intact on an ethidium bromide-stained agarose gel to verify the absence of visible Endonuclease.

Absence of Nickase

1μg of Type I supercoiled pBR322 is incubated with 200 units of Ribonuclease Inhibitor for 4 hours at 37°C.
Following incubation, the supercoiled DNA is visualized on an ethidium bromide-stained agarose gel to verify the absence of visible nicking or cutting.

Absence of Exonuclease

1μg of Lambda DNA/HindIII Markers is incubated with 200 units of Ribonuclease Inhibitor for 16 hours at 37°C.
Following incubation, Lambda DNA/HindIII Markers is separated by 1% agarose gel and stained with ethidium bromide. Markers remain as intact bands without smearing.

Absence of RNase and latent RNase

To test for the presence of RNase activity, 1ug of RNA is incubated with 200 units of Ribonuclease Inhibitor for 4 hours at 37°C.
Following incubation, the RNA is visualized as intact band on an ethidium bromide-stained agarose gel to verify the absence of visible RNase.
To test for the presence of latent RNase activity, 200units of Ribonuclease Inhibitor is heat-denatured at 70°C for 15minutes and incubated with 1ug of RNA for 4 hours at 37°C.
Following incubation, the RNA is visualized as intact band on an ethidium bromide-stained agarose gel to verify the absence of visible RNase.

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recombinant Human Placenta Ribonuclease Inhibitor, 40U/μl

recombinant Murine Ribonuclease Inhibitor, 40U/μl

recombinant Murine Ribonuclease Inhibitor, GMP Grade, for medical mRNA synthesis and other biopharmaceutical production. Size: 40U/μl*75ul/vial

Ribonuclease Inhibitor

RNase inhibitor 40U ul

Product Description

Quality Control

Absence of Endonuclease

Absence of Nickase

Absence of Exonuclease

Absence of RNase and latent RNase

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Tinzyme Co., Limited

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